Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor

 http://repository.vnu.edu.vn/handle/VNU_123/32745


The selectivedetectionof ultratrace amounts of aflatoxinM1(AFM1) is extremely important for food safety since it is themost toxicmycotoxinclass that is allowed tobepresent oncowmilkwith strictly lowregulatory levels. In this work, Fe3O4incorporated polyaniline (Fe3O4/PANi)film has been polymerized on interdigitated electrode (IDE) as sensitivefilm for AFM1 electrochemical biosensor. The immobilized aptamers as an affinity capture reagent and magnetic nanoparticles for signal amplification element have been employed in the sensing platform. Label-free and direct detection of the aptamer-AFM1 on Fe3O4/PANi interface were performed via electrochemical signal change, acquired by cyclic and square wave voltammetries. With a simplified strategy, this electrochemical aptasensor shows a good sensitivity toAFM1 in the range of 6–60 ng·L −1 , with the detection limit of 1.98 ng·L −1 . The results open up the path for designing cost effective aptasensors for other biomedical applications